It accepts one or multiple gene identifiers and returns a number of pre-selected guides from various genome-wide libraries as well as primers with overhangs for cloning into guide RNA expression plasmids and with sequence information preformatted for easy ordering from oligo-array manufacturers.Īdditional assistants in CRISPOR design primers for all predicted off-targets of a guide and create oligonucleotide pools for saturating mutagenesis of the input sequence.īelow, we provide a step by step manual of CRISPOR and instructions on how to use the assistants. For each guide sequence, various primers are designed, e.g. to amplify the target, produce guide RNAs by in vitro transcription after annealing of overlapping primers or for cloning into AddGene plasmids.Ī second, optional mode, called CRISPOR Batch, is available for users who want to use pre-selected guide RNAs for gene inactivation experiments in mouse or human cells. CRISPOR then lists the guides in the input sequence, adding information about them found in databases and algorithms, including genome variants, predicted off-target and on-target scores. In its default mode, the user pastes an input DNA sequence and chooses the genome. CRISPOR saturating mutagenesis assistantĬomments or questions? Email us at What is CRISPOR?ĬRISPOR is a website that helps select and express CRISPR guide sequences, described in two papers ( Gen Biol 2016 and NAR 2018).Maximilian Haeussler, Jean-Paul Concordet
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